Braff, 34, wrote on his Facebook page that the series will not be returning next year.
"Many of you have asked, so here it is: it appears that 'New Scrubs', 'Scrubs 2.0', 'Scrubs with new kids', 'Scrubbier', 'Scrubs without JD' is no more. It was worth a try, but alas ... it didn't work," Braff wrote, signing the entry "zb."
ABC would not comment on the series' future, but chances for a 10th season appear slim considering declining ratings. The season finale drew 3.4 million viewers on March 17.
Season 9 marked a turning point for the series: Many of the show's regular cast members chose not to return. Donald Faison and John C. McGinley still star on the series, but Braff and Sarah Chalke have made only guest appearances. Braff still serves as an executive producer.
Besides casting changes, the focus of Scrubs also shifted from a hospital to a medical school, where Faison and McGinley's characters are both teachers.
Speculation on the series' fate heated up last month when Faison was cast in the CBS pilot The Odds.
Scrubs premiered on NBC in 2001 before moving to ABC in 2009. Series creator and executive producer Bill Lawrence also executive-produces Cougar Town, which ABC picked up for a second season in January.
Do you think Scrubs will return next season?