Bullock, who has yet to comment publicly on James' reported infidelity, broke her silence to refute a report that James has a graphic sex tape, which he could possibly use as leverage in a divorce case.
"There is no sex tape," Bullock said in a statement to People. "There never has been one and there never will be one."
James, 40, reportedly cheated on Bullock, 45, with Michelle "Bombshell: McGee. James apologized to Bullock after the first report appeared, and at least three other women have since come forward claiming to have had affairs with the former Monster Garage star.
Calls and emails to Bullock's rep were not immediately returned.