The actress, who has been mostly absent from the screen scene since the birth of her two children with the TV star, reportedly filed for divorce from Dane on Friday after nearly 14 years of marriage.
Gayheart had been a popular teen slasher star in the late '90s, with starring roles in Jawbreaker and Urban Legend. Meanwhile, Dane rose to mega fame as Dr. Mark Sloan, aka "McSteamy," on Grey's Anatomy and went on to star in The Last Ship.
The pair have made headlines several times in recent years for private matters, including the 2009 release of home video footage showing the two engaging in a sexual encounter with a third party, along with Dane's subsequent struggle with depression.
Gayheart will reportedly seek spousal support along with joint legal and physical custody of the pair's children.