Sit down Castiel. Back off Crowley. The real bromance of Supernatural is between real blood brothers Sam (Jared Padalecki) and Dean (Jensen Ackles). Is that cheating? Maybe. Are they a little too co-dependent? Yes. But no other bros on this list have risked it all (and died) to save one another while also fighting off the forces of evil to save the world.
Photo Credits: BBC; Guy D'Alema, Freeform; Michael Yarish/Getty Images; Take Five, Hulu; Jackson Lee Davis/AMC; Virginia Sherwood/NBC/Warner Brothers; Noam Galai/Getty Images for New York Comic; ABC; Koury Angelo; Amazon