"After 19 years of marriage, Marg and Alan have decided to take some time apart," the actress' rep told People. "They love and respect each other and remain committed to their family."
The couple has an 18-year-old son together.
In addition to her CSI role, Helgenberger will voice the role of Princess Tascela in the movie Conan: Red Nails. Rosenberg, who also had a brief stint on CSI, may be on the cusp of helming an actors' guild strike in the face of an impasse in contract negotiations.