Paltrow will reprise her role as substitute teacher Holly Holiday on the Fox hit, the Oscar winner told Access Hollywood on Sunday.
The 38-year-old actress, who was promoting her new film Country Strong, called her Glee role "the best job ever." No word yet on when the episode will tape or air.
During her guest spot on the musical dramedy, Paltrow performed a rendition of Cee Lo's "Forget You" as well as a mash-up of Rihanna's "Umbrella" and the classic "Singing in the Rain."
An e-mail to the show rep was not immediately returned.
What do you hope Gwyneth will sing for her second go-round?