"We regret that our marital relationship has come to an end, and we have agreed not to make any other public statement on this subject," spokesperson Lisa Linden said in a statement on behalf of the pair.
Spitzer, 54, resigned from office in 2008 after his publicized prostitution scandal. Despite being disgraced, his wife remained by his side. His "mea culpa" press conference is thought to be an inspiration for the CBS series The Good Wife.
The couple has three children together.